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KMID : 0545120130230060878
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Journal of Microbiology and Biotechnology
2013 Volume.23 No. 6 p.878 ~ p.884
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In Vitro Selection of RNA Aptamer Specific to Salmonella Typhimurium
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Han Seung-Ryul
Lee Seong-Wook
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Abstract
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Salmonella is a major foodborne pathogen that causes a variety of human diseases. Development of ligands directly and specifically binding to the Salmonella will be crucial for the rapid detection of, and thus for efficient protection from, the virulent bacteria. In this study, we identified a RNA aptamer-based ligand that can specifically recognize Salmonella Typhimurium through SELEX technology. To this end, we isolated and characterized an RNase-resistant RNA aptamer that bound to the OmpC protein of Salmonella Typhimurium with high specificity and affinity (Kd ~ 20 nM). Of note, the selected aptamer was found to specifically bind to Salmonella Typhimurium, but neither to Gram-positive bacteria (Staphylococcus aureus) nor to other Gram-negative bacteria (Escherichia coli O157:H7). This was evinced by aptamer-immobilized ELISA and aptamer-linked precipitation experiments. This Salmonella species-specific aptamer could be useful as a diagnostic ligand against pathogen-caused foodborne sickness.
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KEYWORD
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OmpC, RNA aptamer, Salmonella typhimurium, SELEX
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